Research Journal of Science and Technology

Tara Chand ¹, Fanish K. Pandey ², Manoj K. Sharma ³

Affiliations
1 CMJ University, Meghalaya, IN
2 Noida International University, Noida, IN
3 Dabur India Limited, Ghaziabad, IN

Abstract

At present almost all the pectinolytic enzymes used for industrial applications are produced by fungi. In this study we produce pectinase from biowaste (orange peels) by solid state fermentation using Aspergillus niger at 30°C and pH 4.5. Production of this enzyme was affected by nature of solid substrate, level of moisture content, presence or absence of carbon, nitrogen, mineral and vitamin supplements to obtain the maximum yield of Pectinase. Maximum enzyme production was obtained from dry substrate in the comparison to wet substrate supplemented with other enzymes like cellulase, amylase in small amount with water at 75% moisture level, after 72hrs of incubation at 30°C. In this work 60% Pectinase was obtained from dry biomass (orange peels).

Keywords

Solid-State Fermentation, A. niger, Orange Peels, Pectinase.

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Tara Chand ¹, Fanish K. Pandey ², Shruti Dhingra ³, Manoj K. Sharma ⁴

Affiliations
1 CMJ University, Meghalaya, IN
2 Noida International University, Greater Noida, IN
3 Delhi University, New Delhi, IN
4 Dabur India Ltd., Ghaziabad, IN

Abstract

In this study the production of cellulases from Aspergillus niger by solid state fermentation was performed using bio wastes viz; sugarcane bagasse and wheat bran. One factor at a time approach was used to optimize the variables such as pH, substrate ratio and temperature. A maximum of 988.2 IU/gm activity of enzyme is observed at substrates ratio of 2:1 (sugarcane bagasse: wheat bran) at 30°C and pH 5 after 5 days incubation. The high cost of production of Cellulotic enzymes has hindered the industrial application of cellulose bioconversion. Hence, this study showed an effective utilization of sugarcane bagasse for the production of cellulotic enzymes which could be effectively used in degradation of cellulose into smaller glucose units in a cost effective manner.

Keywords

Sugarcane Bagasse, Wheat Bran, Aspergillus niger, Cellulase, Cellulose.

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